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lentiviral vector tet plko neo  (Addgene inc)


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    Addgene inc lentiviral vector tet plko neo
    Lentiviral Vector Tet Plko Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+vector+tet+plko+neo/Tet-pLKO-neo+(Plasmid+%2321916)/pmc11259171-281-21-24
    Average 94 stars, based on 65 article reviews
    lentiviral vector tet plko neo - by Bioz Stars, 2026-10
    94/100 stars

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    shRNA:

    Article Title: Huntingtin is an RNA binding protein and participates in NEAT1 -mediated paraspeckles
    Article Snippet: .. HTT shRNA pNT153 and pNT154 from TRCN and negative control shGFP from H. He’s laboratory, sequences listed below, were cloned into lentiviral vector Tet-pLKO-neo (Addgene, #21916) using D. Wiederschain’s protocol (reference: The “all-in-one” system for the inducible expression of shRNA, Novartis Developmental and Molecular Pathways, Cambridge, MA, USA). .. To produce lentivirus, shRNA and packaging plasmids were cotransfected into HEK293 cells using X-tremeGene HP DNA Transfection Reagent (Millipore, catalog no. 6366236001).

    Negative Control:

    Article Title: Huntingtin is an RNA binding protein and participates in NEAT1 -mediated paraspeckles
    Article Snippet: .. HTT shRNA pNT153 and pNT154 from TRCN and negative control shGFP from H. He’s laboratory, sequences listed below, were cloned into lentiviral vector Tet-pLKO-neo (Addgene, #21916) using D. Wiederschain’s protocol (reference: The “all-in-one” system for the inducible expression of shRNA, Novartis Developmental and Molecular Pathways, Cambridge, MA, USA). .. To produce lentivirus, shRNA and packaging plasmids were cotransfected into HEK293 cells using X-tremeGene HP DNA Transfection Reagent (Millipore, catalog no. 6366236001).

    Clone Assay:

    Article Title: Huntingtin is an RNA binding protein and participates in NEAT1 -mediated paraspeckles
    Article Snippet: .. HTT shRNA pNT153 and pNT154 from TRCN and negative control shGFP from H. He’s laboratory, sequences listed below, were cloned into lentiviral vector Tet-pLKO-neo (Addgene, #21916) using D. Wiederschain’s protocol (reference: The “all-in-one” system for the inducible expression of shRNA, Novartis Developmental and Molecular Pathways, Cambridge, MA, USA). .. To produce lentivirus, shRNA and packaging plasmids were cotransfected into HEK293 cells using X-tremeGene HP DNA Transfection Reagent (Millipore, catalog no. 6366236001).

    Expressing:

    Article Title: Huntingtin is an RNA binding protein and participates in NEAT1 -mediated paraspeckles
    Article Snippet: .. HTT shRNA pNT153 and pNT154 from TRCN and negative control shGFP from H. He’s laboratory, sequences listed below, were cloned into lentiviral vector Tet-pLKO-neo (Addgene, #21916) using D. Wiederschain’s protocol (reference: The “all-in-one” system for the inducible expression of shRNA, Novartis Developmental and Molecular Pathways, Cambridge, MA, USA). .. To produce lentivirus, shRNA and packaging plasmids were cotransfected into HEK293 cells using X-tremeGene HP DNA Transfection Reagent (Millipore, catalog no. 6366236001).



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    lentiviral vector tet plko neo  (Addgene inc)
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    Addgene inc lentiviral vector tet plko neo
    Lentiviral Vector Tet Plko Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+vector+tet+plko+neo/Tet-pLKO-neo+(Plasmid+%2321916)/pmc11259171-281-21-24
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    lentiviral vector plko tet on  (Addgene inc)
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    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable <t>lentiviral</t> expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.
    Lentiviral Vector Plko Tet On, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+vector+tet+plko+neo/Tet-pLKO-neo+(Plasmid+%2321916)/pmc09070473-40-0-3
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    lentiviral vectors tet plko neo  (Addgene inc)
    94
    Addgene inc lentiviral vectors tet plko neo
    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable <t>lentiviral</t> expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.
    Lentiviral Vectors Tet Plko Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+vector+tet+plko+neo/Tet-pLKO-neo+(Plasmid+%2321916)/pmc05342093__oncotarget___07___71466___s001-11-9-28
    Average 94 stars, based on 1 article reviews
    lentiviral vectors tet plko neo - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Journal: NAR Cancer

    Article Title: Integrative genome-wide analysis reveals EIF3A as a key downstream regulator of translational repressor protein Musashi 2 (MSI2)

    doi: 10.1093/narcan/zcac015

    Figure Lengend Snippet: Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Article Snippet: Lentiviral vector pLKO-Tet-On (Addgene plasmid #21916) was used to generate inducible knockdown clones of MSI2 and EIF3A in the K562 cell line ( ) (details are provided in the Supplementary Methods).

    Techniques: Knockdown, Western Blot, Expressing, Control, shRNA